Volume 28, Issue 3 (8-2025)
J Arak Uni Med Sci 2025, 28(3): 191-196 |
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Ebadimanas G. The Effect of Mancozeb on Histomorphometric Changes in the Ovaries of Small White Laboratory Mice. J Arak Uni Med Sci 2025; 28 (3) :191-196
URL: http://jams.arakmu.ac.ir/article-1-7859-en.html
URL: http://jams.arakmu.ac.ir/article-1-7859-en.html
Assistant Professor, Department of Biology Education, Farhangian University, Tehran, Iran , ebadimanas@gmail.com
Abstract: (697 Views)
Introduction: Mancozeb a widely used fungicide, is extensively applied to protect fruits, vegetables, and crops. It has various toxic effects on humans. The aim of the present study was to investigate the effects of mancozeb exposure on the ovaries of small laboratory mice.
Methods: Twenty-four immature small white laboratory mice were randomly divided into a control and a mancozeb group, each comprising 12 mice. Each group was divided into three subgroups of days 17, 24, and 31. Mice in the mancozeb group received 100 mg/kg/day mancozeb via gavage for 14 days, and the control group received an equivalent volume of distilled water daily. On day 28, mice were anesthetized for ovary collection, and microscopic sections were prepared and stained from the ovaries. Histomorphometric examination was performed by counting and measuring follicles.
Results: Findings showed that mancozeb exposure resulted in a decrease in the number of mature follicles, an increase in immature follicles, and a reduction in follicle diameter in the experimental group compared to the control group.
Conclusions: Mancozeb disrupts follicle development, potentially leading to reduced fertility in female mice.
Methods: Twenty-four immature small white laboratory mice were randomly divided into a control and a mancozeb group, each comprising 12 mice. Each group was divided into three subgroups of days 17, 24, and 31. Mice in the mancozeb group received 100 mg/kg/day mancozeb via gavage for 14 days, and the control group received an equivalent volume of distilled water daily. On day 28, mice were anesthetized for ovary collection, and microscopic sections were prepared and stained from the ovaries. Histomorphometric examination was performed by counting and measuring follicles.
Results: Findings showed that mancozeb exposure resulted in a decrease in the number of mature follicles, an increase in immature follicles, and a reduction in follicle diameter in the experimental group compared to the control group.
Conclusions: Mancozeb disrupts follicle development, potentially leading to reduced fertility in female mice.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2024/10/29 | Accepted: 2025/05/19
Received: 2024/10/29 | Accepted: 2025/05/19
References
1. Runkle J, Flocks J, Economos J, et al. A systematic review of MCZ as a reproductive and developmental hazard. Environmental International.2017; 99:.29–42.
2. European Food Safety Authority (EFSA), Abdourahime H, Anastassiadou M, Arena M, Auteri D, Barmaz S, Brancato A, Bura L, Carrasco Cabrera L, Chaideftou E, Chiusolo A. Peer review of the pesticide risk assessment of the active substance mancozeb. EFSA Journal. 2020 Dec;18(12):e05755.
3. Yin DaChuan YD, Qi JinYu QJ, Deng YuXia DY, Gao GuoPing GG, Du Hui DH, Chen FangZheng CF, Deng Xun DX. Inhibiting mechanism of two fungicides on Rhizoctonia solani. Journal of Shenyang Agricultural University.2017.
4. Morales-Ovalles Y, Miranda-Contreras L, Peña-Contreras Z, Dávila-Vera D, Balza-Quintero A, Sánchez-Gil B, Mendoza-Briceño RV. Developmental exposure to mancozeb induced neurochemical and morphological alterations in adult male mouse hypothalamus. Environmental Toxicology and Pharmacology. 2018 Dec 1; 64:139-46.
5. Bano F, Mohanty B. Thyroid disrupting pesticides mancozeb and fipronil in mixture caused oxidative damage and genotoxicity in lymphoid organs of mice. Environmental Toxicology and Pharmacology. 2020 Oct 1; 79:103408.
6. Perocco P, Alessandra Santucci M, Campani AG, Forti GC. Toxic and DNA‐damaging activities of the fungicides mancozeb and thiram (TMTD) on human lymphocytes in vitro. Teratogenesis, carcinogenesis, and mutagenesis. 1989; 9(2):75-81.
7. Gatti M, Belli M, De Rubeis M, Khalili MA, Familiari G, Nottola SA, Macchiarelli G, Hajderi E, Palmerini MG. Ultrastructural evaluation of mouse oocytes exposed in vitro to different concentrations of the fungicide Mancozeb. Biology. 2023 May 10; 12(5):698.
8. Yu LI, Ya-Long WA, He SW, Ming-Huang CH, Zhang Z, Xian-Pei FU, Bin-Bin FU, Bao-Qiong LI, Yan-Hong LI, Qi ZQ, Wang HL. Protective effects of resveratrol against mancozeb induced apoptosis damage in mouse oocytes. Oncotarget. 2016 Dec 21;8(4):6233-45.
9. Akthar I, Wang Z, Wijayagunawardane MP, Ratnayake CJ, Siriweera EH, Lee KF, Kodithuwakku SP. In vitro and in vivo impairment of embryo implantation by commonly used fungicide Mancozeb. Biochemical and Biophysical Research Communications. 2020 Jun 18;527(1):42-8.
10. Arbuckle TE, Lin Z, Mery LS. An exploratory analysis of the effect of pesticide exposure on the risk of spontaneous abortion in an Ontario farm population. Environmental health perspectives. 2001 Aug;109(8):851-7.
11. Bianchi S, Nottola SA, Torge D, Palmerini MG, Necozione S, Macchiarelli G. Association between female reproductive health and mancozeb: Systematic review of experimental models. International Journal of Environmental Research and Public Health. 2020 Apr;17(7):2580.
12. Dall'Agnol JC, Pezzini MF, Uribe NS, Joveleviths D. Systemic effects of the pesticide mancozeb-A literature review. European Review for Medical & Pharmacological Sciences. 2021 Jun 1;25(11).
13. Kwon D, Chung HK, Shin WS, Park YS, Kwon SC, Song JS, Park BG. Toxicological evaluation of dithiocarbamate fungicide mancozeb on the endocrine functions in male rats. Molecular & Cellular Toxicology. 2018 Jan;14:105-12.
14. Suarez Uribe ND, Pezzini MF, Dall’Agnol J, Marroni N, Benitez S, Benedetti D, Da Silva J, Cerski CT, Dallegrave E, Macedo S, De Oliveira SC. Study of liver toxicity and DNA damage due to exposure to the pesticide Mancozeb in an experimental animal model–A pilot model. European Review for Medical & Pharmacological Sciences. 2023 Jul 1;27(13).
15. Bao J, Zhang Y, Wen R, Zhang L, Wang X. Low level of mancozeb exposure affects ovary in mice. Ecotoxicology and Environmental Safety. 2022 Jul 1;239:113670.
16. Joshi SC, Gulati N, Gajraj A. Evaluation of toxic impacts of mancozeb on testis in rats. Asian Journal of Experimental Sciences. 2005; 19(1): 73-83.
17. Hoshyari A, Najafi G, Sadrkhanlo R. & Zarei L .Effects of Ruta graveolens (RG) aqueous extract on diameter and number of different ovarian follicles in mice.2015. Studies in Medical Sciences, 26(6), pp.504–12.
18. Girish BP, Reddy PS. Forskolin ameliorates mancozeb‐induced testicular and epididymal toxicity in Wistar rats by reducing oxidative toxicity and by stimulating steroidogenesis. Journal of Biochemical and Molecular Toxicology. 2018 Feb;32(2):e22026.
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