Volume 29, Issue 1 (3-2026)
J Arak Uni Med Sci 2026, 29(1): 5-12 |
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Nanava Z, Hatami Nemati H, Ahmai H, Khakpay R. Study on Changes in the Expression of Histamine and Histamine N-Methyltransferase Genes in the Lumbar Spinal Cord of Male Rats during Methamphetamine Abuse and Buprenorphine Treatment. J Arak Uni Med Sci 2026; 29 (1) :5-12
URL: http://jams.arakmu.ac.ir/article-1-8046-en.html
URL: http://jams.arakmu.ac.ir/article-1-8046-en.html
1- MSc, Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
2- Associate Professor, Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
3- Assistant Professor, Department of Basic Sciences, Farhangian University, P.O. Box 14665- 889 Tehran, Iran ,hahmadi@cfu.ac.ir
2- Associate Professor, Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
3- Assistant Professor, Department of Basic Sciences, Farhangian University, P.O. Box 14665- 889 Tehran, Iran ,
Abstract: (487 Views)
Introduction: Methamphetamine is a powerful psychostimulant which has been significantly abused in recent years. Buprenorphine, a derivative of morphine alkaloids, is effective in treating opioid addiction.
Methods: This experimental study involved eight groups of seven male rats each. It examined the effects of a 5-day intraperitoneal injection of methamphetamine, buprenorphine, their interactions, and methamphetamine withdrawal on the expression of histamine and histamine N-methyltransferase genes in the lumbar spinal cord. The collected data were analyzed using One-way ANOVA.
Results: The intraperitoneal administration of 10 mg/kg of methamphetamine and 6 or 10 mg/kg of buprenorphine over five days did not change the expression levels of histamine or histamine N-methyltransferase genes in of male rats lumbar spinal cord. However, discontinuing methamphetamine led to an increase in the expression of both genes in this area (P < 0.01). Furthermore, when examining the interaction between the two drugs, it was found that the expression of histamine N-methyltransferase gene was significantly higher in the group receiving methamphetamine plus 10 mg/kg buprenorphine compared to the methamphetamine-only group (P < 0.01).
Conclusions: Based on the results of this study and the mechanisms proposed in previous studies, it seems that methamphetamine withdrawal and/or the use of buprenorphine as a possible therapeutic modality can lead to stabilization of physiological balance in central nervous system by temporarily increasing brain histamine, thus reducing the complications of methamphetamine abuse.
Methods: This experimental study involved eight groups of seven male rats each. It examined the effects of a 5-day intraperitoneal injection of methamphetamine, buprenorphine, their interactions, and methamphetamine withdrawal on the expression of histamine and histamine N-methyltransferase genes in the lumbar spinal cord. The collected data were analyzed using One-way ANOVA.
Results: The intraperitoneal administration of 10 mg/kg of methamphetamine and 6 or 10 mg/kg of buprenorphine over five days did not change the expression levels of histamine or histamine N-methyltransferase genes in of male rats lumbar spinal cord. However, discontinuing methamphetamine led to an increase in the expression of both genes in this area (P < 0.01). Furthermore, when examining the interaction between the two drugs, it was found that the expression of histamine N-methyltransferase gene was significantly higher in the group receiving methamphetamine plus 10 mg/kg buprenorphine compared to the methamphetamine-only group (P < 0.01).
Conclusions: Based on the results of this study and the mechanisms proposed in previous studies, it seems that methamphetamine withdrawal and/or the use of buprenorphine as a possible therapeutic modality can lead to stabilization of physiological balance in central nervous system by temporarily increasing brain histamine, thus reducing the complications of methamphetamine abuse.
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