Volume 15, Issue 8 (January 2013)                   J Arak Uni Med Sci 2013, 15(8): 16-25 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Behzadian F, Goodarzi Z, Saberfar E. Construction of a new recombinant baculovirus encoding HA, NA, and M1 proteins of swine influenza (H1N1) virus and its expression in insect cells. J Arak Uni Med Sci. 2013; 15 (8) :16-25
URL: http://jams.arakmu.ac.ir/article-1-1495-en.html
1- research senter for sciences and biotechnology, research senter for sciences and biotechnology,tehran, iran, p.o.box 15875-1774
2- applied virology research center, applied virology research center, baqyatallah univesity of medical sciences , tehran, iran
3- applied virology research center , saberfar@yahoo.com
Abstract:   (7690 Views)
Background: Genetic variability of influenza viruses causes new epidemics worldwide annually. Development of a new vaccine for prophylaxis of influenza virus has been amajor objective in recent years. The aim of this study was to construct a recombinant baculoviruscapable of expressing the two surficial antigenic glycoproteins, hemagglutininand neuraminidase, as well as matrix proteinsof swine influenza (H1N1) simultaneously and independently. Materials and Methods: In this experimental study, first, a triplet cassette providing simultaneous and independent expression of target proteins was designed and subjected to synthesis. It was then cloned into pFastBac1 donor plasmid. Competent E.ColiDH10Bac cells were transformed by donor clone and the recombinant bacmids were produced following homologous transposition. This construction was verified by PCR and then transfected into Sf9 insect cells to package new recombinant baculoviruses. Results: Restriction map of pFastBacI HNM1 donor plasmid confirmed the fidelity of the clone. The results of PCR done on the recombinant bacmidas template indicated that a proper homologous recombination has occurred between pFastBacI HNM1 donor plasmid and the bacmid in E.ColiDH10Bac host cells. Protein analysis of the infected Sf9 cells showed that all target proteins were efficiently expressed at the same time. Conclusion: The recombinant baculovirus constructed in this studypossesses proper characteristics to produce swine influenza virus-like particles in Sf9 cells.
Full-Text [PDF 298 kb]   (1787 Downloads)    
Type of Study: Original Atricle | Subject: Basic Sciences
Received: 2012/01/2 | Accepted: 2012/05/14
* Corresponding Author Address: applied virology research center, baqyatallah univesity of medical sciences , tehran, iran

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2019 All Rights Reserved | Journal of Arak University of Medical Sciences

Designed & Developed by : Yektaweb