Volume 22, Issue 1 (4-2019)
J Arak Uni Med Sci 2019, 22(1): 27-38 |
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Parvin A, Yaghmaei P, Noureddini M, Haeri Roohani A, Aminzadeh S. Comparative Investigation of the Effect of Quercetin and Hydroalcoholic Extract of Otostegia Persica Boiss with Atorvastatin on ABC A1 Gene Expression in Hypercholesterolemic Male Wistar Rats. J Arak Uni Med Sci 2019; 22 (1) :27-38
URL: http://jams.arakmu.ac.ir/article-1-5799-en.html
URL: http://jams.arakmu.ac.ir/article-1-5799-en.html
1- Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- Department of Applied Cell Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran. ,mnoureddini@yahoo.com
3- Department of Bioprocess Engineering Research, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
2- Department of Applied Cell Sciences, Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran. ,
3- Department of Bioprocess Engineering Research, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Abstract: (2961 Views)
Background and Aim: Hypercholesterolemia is an independent risk factor for atherosclerosis that the use of medicinal plants with minimal side effects is very important in the treatment of it. In this study, comparative evaluation of the effect of hydroalcoholic extract and quercetin of Otostegia persica Boiss with atorvastatin on ABC A1 gene expression in hypercholesterolemic male Wistar rats was carried out.
Materials and Methods: Forty male wistar rats with about 180gr weight randomly individed into five groups of eight: 3 experimental groups, 1 sham group and 1 control group. The experimental and sham groups received a high-fat diet with 2% cholesterol (through gavage) for 40 days. The experimental groups were treated (were fed) separately with 40 mg/kg/day atorvastatin, 25 mg/kg/day quercetin and 25 mg/kg/day hydroalcoholic extract of Otostegia persica Boiss for 28 days. Sham group received daily 1 mg/kg saline water during this period. In the end, the expression of ABC A1 gene was determined by Real-Time PCR in leukocytes and serum lipids were measured by photometric method.
Ethical Considerations: This study with research ethics code B/29/5/1/1799 has been approved by committee for ethics in biomedical research at Kashan university of medical sciensec on July 31, 2016.
Findings: The hydroalcoholic extract and quercetin of Otostegia persica Boiss and atorvastatin significantly increased ABC A1 gene expression in three experimental groups {(1.14 ± 0.09) ,(1.18 ± 0.03),(1.11 ± 0.03) respectively} realated to control group(1.00 ± 0.011) (p<0.05) and sham group(0.89 ± 0.03) (p<0.05). Quercetin also was more effective than atorvastatin in increasing of ABC A1 gene expression (p<0.05).
Conclusion: Quercetin and hydroalcoholic extract of Otostegia persica Boiss have increased effect on ABC A1 gene expression in hypercholesterolemic male Wistar rats.
Materials and Methods: Forty male wistar rats with about 180gr weight randomly individed into five groups of eight: 3 experimental groups, 1 sham group and 1 control group. The experimental and sham groups received a high-fat diet with 2% cholesterol (through gavage) for 40 days. The experimental groups were treated (were fed) separately with 40 mg/kg/day atorvastatin, 25 mg/kg/day quercetin and 25 mg/kg/day hydroalcoholic extract of Otostegia persica Boiss for 28 days. Sham group received daily 1 mg/kg saline water during this period. In the end, the expression of ABC A1 gene was determined by Real-Time PCR in leukocytes and serum lipids were measured by photometric method.
Ethical Considerations: This study with research ethics code B/29/5/1/1799 has been approved by committee for ethics in biomedical research at Kashan university of medical sciensec on July 31, 2016.
Findings: The hydroalcoholic extract and quercetin of Otostegia persica Boiss and atorvastatin significantly increased ABC A1 gene expression in three experimental groups {(1.14 ± 0.09) ,(1.18 ± 0.03),(1.11 ± 0.03) respectively} realated to control group(1.00 ± 0.011) (p<0.05) and sham group(0.89 ± 0.03) (p<0.05). Quercetin also was more effective than atorvastatin in increasing of ABC A1 gene expression (p<0.05).
Conclusion: Quercetin and hydroalcoholic extract of Otostegia persica Boiss have increased effect on ABC A1 gene expression in hypercholesterolemic male Wistar rats.
Keywords: ABC A1 gene, Atorvastatin, Hydroalcoholic extract, Hypercholesterolemia, Otostegia persica Boiss, Quercetin
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2018/06/7 | Accepted: 2018/09/17
Received: 2018/06/7 | Accepted: 2018/09/17
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