Volume 14, Issue 7 (Brucellosis Supplement 2012)
J Arak Uni Med Sci 2012, 14(7): 1-8 |
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Abtahi H, Salmanian A H, Rafati S, Mossayebi G, Amouzande A R. Evaluation of the immunogenicity of Brucella abortus P39 gene in Balb/c mice. J Arak Uni Med Sci 2012; 14 (7) :1-8
URL: http://jams.arakmu.ac.ir/article-1-1352-en.html
URL: http://jams.arakmu.ac.ir/article-1-1352-en.html
1- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran , h_abtahi2@yahoo.co.uk
2- National Research Center for Genetic Engineering and Biotechnology, Tehran, I.R.Iran.
3- Dept. Of Immunology, Pasteur Institute of Iran, Tehran.
4- Molecular and Medicine Research Center , Arak University of Medical Sciences, Arak, Iran
5- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran
2- National Research Center for Genetic Engineering and Biotechnology, Tehran, I.R.Iran.
3- Dept. Of Immunology, Pasteur Institute of Iran, Tehran.
4- Molecular and Medicine Research Center , Arak University of Medical Sciences, Arak, Iran
5- Molecular and Medical Research Center, Arak University of Medical Sciences, Arak, Iran
Abstract: (12678 Views)
Background: Brucella is a gram-negative intracellular bacterium. Since Brucella brings about health and socio-economic problems, its control is of primary importance. The common method of vaccination includes using live attenuated strains of this bacterium. This study was done to evaluate the immunogenicity of Brucella aburtus P39 gene in Balb/c mice.
Materials and Methods: In this experimental study, P39 gene was amplified by polymerase chain reaction (PCR) method and after extraction, it was sub-cloned to eukaryotic expression vector pcDNA3. The intramuscular injection of the obtained plasmid to the Balb/c mice was done in three stages. After the last vaccination, immunologic tests, such as proliferative response in lymphocytes, IFN- assessment, IL-5, and determination of IgG2a and IgG1, were run.
Results: The level of activation in splenic lymphocytes response was 3.6 and the measured IFN- was 3 ng/ml, whereas IL-5 was insignificant. IgG2a and IgG1 titers were 1.640 and 1.40, respectively.
Conclusion: The findings of the immunological analysis show the appropriate immune response in Balb/c mice model after the injection of P39 gene containing plasmid. The immune system response was in Th1 form which decreased the number of bacteria in spleen. Therefore, P39 gene is of appropriate immunogenicity and DNA vaccination is efficient in the activation of cell immune response against this bacterium.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2011/10/9 | Accepted: 2011/12/29
Received: 2011/10/9 | Accepted: 2011/12/29
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