Volume 19, Issue 4 (7-2016)                   J Arak Uni Med Sci 2016, 19(4): 1-10 | Back to browse issues page

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Heidary M, Bahramian A, Goudarzi H, Eslami G, Hashemi A, Khoshnood S. To Study the Association between AcrAB and Qep A Efflux Pumps and Ciprofloxacin Resistance among Escherichia Coli and Klebsiella Pneumoniae Clinical Strains. J Arak Uni Med Sci 2016; 19 (4) :1-10
URL: http://jams.arakmu.ac.ir/article-1-4155-en.html
1- Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- Department of Microbiology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Abstract:   (5293 Views)

Background: The efflux pumps are one of the main mechanisms of resistance to antibiotics in E. coli and K. pneumoniae strains. The aim of this study is to study the association between genes coding efflux pumps AcrAB and Qep A and ciprofloxacin resistance among E. coli and K. pneumoniae clinical strains

Materials and Methods: This study was done on 100 strains of E. coli isolated from Taleghani and Labbafinejad Hospitals and 100 strains of K. pneumoniae isolated from Taleghani and Mofid Children Hospitals. Antimicrobial susceptibility tests were performed by disk diffusion method based on CLSI guidelines. Identification of genes encoding efflux pumps Acr AB and Qep A was done by PCR technique.

Results: In this study, fosfomycin and imipenem had the best effect against E. coli clinical isolates and fosfomycin and tigecycline had the best effect against K. pneumoniae clinical isolates. PCR assay demonstrated that the prevalence of AcrA, AcrB and QepA genes among E. coli isolates were 92%, 84% and 0%, respectively and among K. pneumoniae isolates were 94%, 87% and 4%, respectively.

Conclusion: The prevalence of genes encoding efflux pumps in E. coli and K. pneumoniae clinical strains, which causes resistance to fluoroquinolones, is cause for concern. Therefore, controlling infection and preventing the spread of drug-resistant bacteria needs to manage medication carefully and identify resistant isolates.

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Type of Study: Original Atricle | Subject: Infection
Received: 2015/12/29 | Accepted: 2016/04/27

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