Volume 21, Issue 5 (10-2018)
J Arak Uni Med Sci 2018, 21(5): 77-87 |
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Rooshenas F, Ashrafi M, Nazifi S, Aminlari M, Talebanzadeh S. Evaluation the Effect of Saffron Aqueous Extract on Oxidative Stress Parameters and Important Biochemical Enzymes of Liver Tissue in Streptozotocin-Induced Diabetic Rats . J Arak Uni Med Sci 2018; 21 (5) :77-87
URL: http://jams.arakmu.ac.ir/article-1-5394-en.html
URL: http://jams.arakmu.ac.ir/article-1-5394-en.html
1- Department of Basic Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran.
2- Department of Clinical Studies, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran. ,nazifi@shirazu.ac.ir
2- Department of Clinical Studies, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran. ,
Abstract: (3090 Views)
Background and Aim: Medicinal plants possessing antioxidant activity may reduce oxidative stress and improve the functions of various organs that affected by hyperglycemia. The aim of the present study was to evaluate the effects of saffron aqueous extract (SAE) administration to diabetic rats by measuring the oxidative stress parameters and important biochemical enzymes in liver tissue.
Materials and Methods: 72 hours after STZ administration (60 mg/kg body weight), the animals with fasting blood glucose over of 250 mg/dl were considered to be diabetic rats and experimental groups were: control (1), control drug (2), diabetes (3) and diabetes drug (4). The treatment was started on the 7th day after STZ injection with i.p injection of SAE (200mg/kg body weight), five doses and weekly to groups 2 and 4. At the end of the experimental period, biochemical factors were measured after bleeding and harvesting of tissues.
Findings: Results indicated the perturbation in the activity of important liver enzymes in diabetic group (3) and SAE adjusted and normalized their levels activity. In addition, SAE with increases in the activity of antioxidant enzymes alleviated diabetes induced oxidative stress and thus reduced MDA levels in group 4 compared to group 3.
Conclusion: SAE is not only useful in the controlling of blood glucose, but also has antioxidant potential to protect the liver tissue of diabetic rats against damage caused by hyperglycemia-induced oxidative stress.
Materials and Methods: 72 hours after STZ administration (60 mg/kg body weight), the animals with fasting blood glucose over of 250 mg/dl were considered to be diabetic rats and experimental groups were: control (1), control drug (2), diabetes (3) and diabetes drug (4). The treatment was started on the 7th day after STZ injection with i.p injection of SAE (200mg/kg body weight), five doses and weekly to groups 2 and 4. At the end of the experimental period, biochemical factors were measured after bleeding and harvesting of tissues.
Findings: Results indicated the perturbation in the activity of important liver enzymes in diabetic group (3) and SAE adjusted and normalized their levels activity. In addition, SAE with increases in the activity of antioxidant enzymes alleviated diabetes induced oxidative stress and thus reduced MDA levels in group 4 compared to group 3.
Conclusion: SAE is not only useful in the controlling of blood glucose, but also has antioxidant potential to protect the liver tissue of diabetic rats against damage caused by hyperglycemia-induced oxidative stress.
Keywords: Biochemical and antioxidant enzymes, Diabetes mellitus, Liver, Saffron aqueous extract, Streptozotocin
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2017/10/20 | Accepted: 2018/05/27
Received: 2017/10/20 | Accepted: 2018/05/27
References
1. Forbes, J. M., & Cooper, M. E. Mechanisms of diabetic complications. Physiological reviews. 2013; 93(1): 137-188.
2. Elgazar, A. F., Rezq, A. A., & Bukhari, H. M. Anti-hyperglycemic effect of saffron extract in alloxan-induced diabetic rats. Eur J Biol Sci. 2013; 5(1): 14-22.
3. Lucchesi, A. N., Freitas, N. T. D., Cassettari, L. L., Marques, S. F. G., & Spadella, C. T. Diabetes mellitus triggers oxidative stress in the liver of alloxan-treated rats: a mechanism for diabetic chronic liver disease. Acta Cirurgica Brasileira. 2013; 28(7): 502-508.
4. Roskams, T., Yang, S. Q., Koteish, A., Durnez, A., DeVos, R., Huang, X., & Diehl, A. M. Oxidative stress and oval cell accumulation in mice and humans with alcoholic and nonalcoholic fatty liver disease. The American journal of pathology. 2003; 163(4): 1301-1311.
5. Aly, H. F., & Mantawy, M. M. Comparative effects of zinc, selenium and vitamin E or their combination on carbohydrate metabolizing enzymes and oxidative stress in streptozotocine-induced diabetic rats. Eur Rev Med Pharmacol Sci. 2012; 16: 66-78.
6. Bathaie, S. Z., & Mousavi, S. Z. New applications and mechanisms of action of saffron and its important ingredients. Critical Reviews in Food Science and Nutrition. 2010; 50(8): 761-786.
7. Mousavi, S. Z., & Bathaie, S. Z. Historical uses of saffron: Identifying potential new avenues for modern research. Avicenna Journal of Phytomedicine. 2011; 1(2): 57-66.
8. Iliass Lahmass, L., Ouahhoud, S., Sabouni, A., Elyoubi, M., Benabbas, R., Elmoussaoui, R., Choukri, M., Saalaoui, E. Antihyperlipidemic effect of crude extract of saffron (Crocus sativus) stigma in healthy male rats. J Med Allied Sci. 2017; 7(1): 20-25.
9. Mehdizadeh, R, Parizadeh, M.R., Khooei, A.R., Mehri, S., Hosseinzadeh, H. Cardioprotective effect of saffron extract and safranal in isoproterenol-induced myocardial infarction in wistar rats. Iran J Basic Med Sci. 2013; 16(1):56-63.
10. Ahmad, A.S., Ansari, M.A., Ahmad, M. Neuroprotection by crocetin in a hemi- parkinsonian rat model. Pharmaco lBiochem Behav. 2005; 81: 805-13.
11. Riaz, H. Hepatoprotective effect of crocus sativuse on amiodarone induced liver toxicity. BJPR. 2016; 12(4): 1-11.
12. Bathaie, S.Z., Ashrafi, M, Azizian M., Tamanoi, F. Recent progress in saffron and its constituents: crocin, crocetin, picrocrocin and safranal. Recent Progress in Medicinal Plants. 2016; 43: 1-29.
13. Shirali, S., Bathaei, S. Z., Nakhjavani, M., & Ashoori, M. R. Effects of saffron (Crocus Sativus L.) aqueous extract on serum biochemical factors in streptozotocin-induced diabetic rats. 2012; 293-308.
14. Peeri, M., Haghigh, M. M., Azarbayjani, M. A., Atashak, S., & Behrouzi, G. Effect of aqueous extract of saffron and aerobic training on hepatic non-enzymatic antioxidant levels in streptozotocin-diabetic rats. Archives Des Sciences. 2012; 65(10): 525-32.
15. Arkkila, P. E., Koskinen, P. J., Kantola, I. M., Rönnemaa, T., Seppänen, E., & Viikari, J. S. Diabetic complications are associated with liver enzyme activities in people with type 1 diabetes. Diabetes Research and Clinical Practice. 2001; 52(2): 113-118.
16. Song, R. H., Singh, A. K., & Leehey, D. J. Decresead glomerular proteinase activity in the STZ-diabetic rats. Am. J. Nephrol. 1996; 19(3): 441-6.
17. Krishnakumari, S., Bhuvaneswari P., and Rajeswari P. Ameliorative potential of Coccinia grandis extract on serum and liver marker enzymes and lipid profile in streptozotocin induced diabetic rats. Anc Sci Life. 2011; 31(1):26-30.
18. Rogers, K.S., Edwin, S., Higgers E.S. Experimental diabetes causes mitochondrial loss and cytoplasmic enrichment of pyridoxal phosphate and aspartate aminotransferase activity. Bioch Met Biol. 1986; 36(1): 91-97.
19. Amouoghli Tabrizi, B., & Mohajeri, D. Protective effect of edible turmeric (Curcuma longa Linn.) powder on early hepatic injury in diabetic rats. Kaums Journal (Feyz). 2010; 14(3): 190-199.
20. Bolkent, S., Sacan, O., Karatug, A., & Yanardag, R. The effects of vitamin B6 on the liver of diabetic rats: A morphological and biochemical study. Iufs J Biol. 2008; 67(1): 1-7.
21. Fernandez, A. A. H., Novelli, E. L. B., Fernandes Junior, A., & Galhardi, C. M. Effect of naringerin on biochemical parameters in the streptozotocin-induced diabetic rats. Brazilian Archives of Biology and Technology. 2009; 52(1): 51-59.
22. Mohammad, R., Daryoush, M., Ali, R., Yousef, D., & Mehrdad, N. Attenuation of oxidative stress of hepatic tissue by ethanolic extract of saffron (dried stigmas of Crocus sativus L.) in streptozotocin (STZ)-induced diabetic rats. African Journal of Pharmacy and Pharmacology. 2011; 5(19): 2166-2173.
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