Volume 21, Issue 4 (8-2018)
J Arak Uni Med Sci 2018, 21(4): 6-17 |
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Zalkhani R, Moazedi A A, Ghotbeddin Z, Pourmahdi Borujeni M. Evaluation the Efficacy of Sodium Valproate and Low Frequency Stimulation During CA1 Hippocampal Kindling on Anxiety-Like Behavior in Adult Male Rat. J Arak Uni Med Sci 2018; 21 (4) :6-17
URL: http://jams.arakmu.ac.ir/article-1-5573-en.html
URL: http://jams.arakmu.ac.ir/article-1-5573-en.html
1- Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2- Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. ,moazedi.a@gmail.com
3- Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
4- Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2- Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. ,
3- Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
4- Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Abstract: (2879 Views)
Background and Aim: Emotional disorders are prevalent in many epileptic patients. So, in this research, we have studied the efficacy of two treatment methods of seizure on anxiety-like behavior during kindling in adult male rat.
Materials and Methods: 42 male rats were randomly divided into 6 groups: Control, Sham operation, saline-kindled and drug-kindled groups which have received saline or drug 15 minutes before kindling stimulations, and saline-kindled-LFS or drug-kindled-LFS group which have received saline or drug 15 minutes before kindling stimulations and LFS applied after termination of kindling stimulations. Anxiety-like behavior was assessed on the 6th day by using elevated plus maze and open field apparatus.
Findings: kindling significantly increased open arms (OAs) entries percentage, OAs exploration percentage, increasing jumping from elevated plus maze (p<0.001) and rearing frequency in open field apparatus (p<0.05) compared to the sham group. Sodium valproate increased OAs entries percentage and OAs exploration percentage in drug-kindled group compared to sham group (p<0.001). But, there wasn’t any significant difference in jumping from elevated plus maze and rearing in open field compared to sham group. Also, there was no significant change in these parameters in Saline-kindled-LFS, drug-kindled and drug–kindled-LFS groups.
Conclusion: Sodium valproate and LFS, as two therapies controlling epilepsy, decrease anxiety induced by kindling stimulation.
Materials and Methods: 42 male rats were randomly divided into 6 groups: Control, Sham operation, saline-kindled and drug-kindled groups which have received saline or drug 15 minutes before kindling stimulations, and saline-kindled-LFS or drug-kindled-LFS group which have received saline or drug 15 minutes before kindling stimulations and LFS applied after termination of kindling stimulations. Anxiety-like behavior was assessed on the 6th day by using elevated plus maze and open field apparatus.
Findings: kindling significantly increased open arms (OAs) entries percentage, OAs exploration percentage, increasing jumping from elevated plus maze (p<0.001) and rearing frequency in open field apparatus (p<0.05) compared to the sham group. Sodium valproate increased OAs entries percentage and OAs exploration percentage in drug-kindled group compared to sham group (p<0.001). But, there wasn’t any significant difference in jumping from elevated plus maze and rearing in open field compared to sham group. Also, there was no significant change in these parameters in Saline-kindled-LFS, drug-kindled and drug–kindled-LFS groups.
Conclusion: Sodium valproate and LFS, as two therapies controlling epilepsy, decrease anxiety induced by kindling stimulation.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2018/01/15 | Accepted: 2018/05/29
Received: 2018/01/15 | Accepted: 2018/05/29
References
1. Mc Namara JO: Cellular and molecular basis of epilepsy, J Neurosci. 1994;14: 3413-3425.
2. Loscher W, Animal models of epilepsy for the development of antiepileptogenic and disease-modifying drugs. A comparison of the pharmacology of kindling and post-status epilepticus models of temporal lobe epilepsy, Epilepsy Res. 2002; 50: 105-123.
3. Loscher W, Current status and future direction in the pharmacotherapy of epilepsy, Trends Pharmacol Sci. 2002; 23: 113-8.
4. Kandrataviciusl L, Balista1 P, Lopes-Aguiar1C, Ruggiero R, Umeoka E, Garcia-Cairasco N, Bueno-Junior1L, Leite J, Animal models of epilepsy: use and limitations, Neuropsychiatr Dis Treat. 2014; 10: 1693–1705.
5. Sander JW, The use of antiepileptic drug-principles and practice, Epilepsia. 2004; 45: 28-34.
6. Shahpari M, Mirnajafizade J, Firoozabadi S, Yadollahpour A, effect of low-frequency electrical stimulation parameters on its anticonvulsant action during rapid performant path kindling in rat, Epilepsy Res. 2012; 99: 69-77.
7. Wyckhuys T, Raedt R, Vonck K, Wadman W, Boon P, Comparison of hippocampal deep brain stimulation with high (130) and low frequency(5Hz) on afterdischarges in kindled rats, Epilepsy Res. 2010; 88: 239-246.
8. Gaito J, Nobrega J.N, Gaito, S.T, Interference effect of 3 Hz brain stimulation on kindling behavior induced by 60 Hz stimulation, Epilepsia.1980; 21:73-84.
9. Silver JM, Shin C, McNamara JO, Antiepileptogeic effects of conventional anticonvulsants in the kindling model of epilepsy, Ann Neurol. 1991; 29: 356-363.
10. Bolanos AR, Sarkisian M. Yang Y, Hori A, Helmers SL, Mikati M, Tandon P, Stafstrom CE, Holmes GL. Comparison of valproate and phenobarbital treatment after status epilepticus in rats, Neurol. 1998; 51: 41-48.
11. Brandt C, Gastens AM, Sun M, Hausknecht M, and Loscher W. Treatment with valproate after status epilepticus: effect on neuronal damage, epileptogenesis and behavioral alteration in rats, Neuropharmacol. 2006; 51:789-804.
12. Klitgaard HV, Matagne AC, Vanneste Goemaere J, and Margineanu DG. Effect of prolonged administration of levetiracetam on pilocarpine-induced epileptogenesis in rats, Epilepsia. 2001; 42:114-115.
13. Asgari A, Semnanian S, Atapour N, Shojaei A, Mirnajafi-zadeh J, combined sub-threshold dosages of phenobarbital and low frequency stimulation effectively reduce seizures in amygdala-kindled rats, Neurol Sci. 2014; 35: 1255-1260.
14. Cuellar-Herrera M, Pena F, Alcantara-Gonzalez D, Neri-Bazan L, Rocha L, Antiepileptic drugs combined with high-frequency electrical stimulation in the ventral hippocampus modify pilocarpine-induced status epilepticus in rats, Epilepsia. 2010; 51: 432-437.
15. Kalynchuk L, Pinel J, Treit D, Kippin T, Changes in emotional behavior produced by long-term amygdala kindling in rats, Biol Psychiatry. 1997; 41: 438-451.
16. Piazzini A, Canevini M, Maggiori M, R. Canger R, Depression and anxiety in patients with epilepsy, Epilepsy Behav. 2001; 2:481-489.
17. Perucca E, Pharmacological and therapeutic properties of valproate, CNS drugs. 2002; 16: 695-714.
18. Jalilifar M, Yadollahpour A, Moazedi A.A, Ghotbeddin Z. Low Frequency electrical stimulation either prior to or after rapid kindling stimulation inhibits the kindling-induced epileptogenesis, Biomed Res Int. 2017; 2017.
19. Racine R, Rose P, Burnham W, Afterdischarge thresholds and kindling rates in dorsal and ventral hippocampus and dentate gyrus, Can J Neurol Sci. 1977; 4: 273-278.
20. Charghan S, Eshagh Harooni H, Moazedi A, Khaje-Pour L, Interaction of zinc chloride with an aromatase inhibitor (letrozole) on anxiety in adult male rats, Arak Medical University Journal (AMUJ). 2016; 19:34-45.
21. Angrini M, Leslie J, Shephard R, Effects of propranolol, buspirone, pCPA, reserpine, and chlordiazepoxide on open-field behavior, Pharmacol Biochem Behav. 1998; 59: 387-397.
22. Kalynchuk L, Pinel J, Treit D, Kippin T. Long-term kindling and interictal emotionality in rats: effect of stimulation site, Brain Res.1998; 41: 149–157.
23. Hannesson D, Howland J, Pollock M, Mohapel P, Wallace A, Corcoran M, Dorsal hippocampal kindling produces a selective and enduring disruption of hippocampally mediated behavior, Jnsci. 2001; 21: 4443-4450.
24. Shang-Der Chen, Yu-Lin Wang, Sheng-Fu Liang,Fu-Zen Shaw, Rapid amygdala kindling causes motor seizure and comorbidity of anxiety and depression-like behaviors in rats, Front Behav Neurosci. 2016; 10: 129.
25. Helfer V, Deransart C, Marescaux, Depaulis A, Amygdala kindling in the rats: Anxiogenic-like consequences, Neurosci. 1996; 73: 971-978.
26. Godlevsky L, Muratova T, Kresyun N, Luijtelaar E, Coenen M, Anxiolytic and antidepressive effects of electric stimulation of the paleocerebellar cortex in pentylenetetrazol kindled rats, Acta Neurobiol Exp. 2014; 74: 456-464.
27. Perucca E, Pharmacological and therapeutic properties of valproate, CNS drugs. 2002; 16:695-714.
28. Sharma AK, Reams RY, Jordan WH, Miller MA, Thacker HL, Snyder PW, Mesial temporal lobe epilepsy: Pathogenesis, induced rodent models and lesions, Toxicol Pathol. 2007; 35: 984–998.
29. Kalynchuk L, Pearson D, Pinel J, Meaney M, Effect of amygdala kindling on emotional behavior and benzodiazepine receptor binding in rats, ANAS877. 1999; 737-741.
30. Buteme J, the role of valproic acid, a histone deacetylase inhibitor, in reducing anxiety levels in rats: an epigenetics study, 2015. Honors College Theses.
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