Volume 22, Issue 3 (8-2019)                   J Arak Uni Med Sci 2019, 22(3): 118-128 | Back to browse issues page

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MalekiPoya M, abedi B, Palizvan M R, Saremi A. The Effect of Eight Weeks of Incremental Endurance Training on The Levels of Matrix Metalloproteinase-1 (MMP1) and Thrombosponidine-1 (TSP1) in the Rats, Induced by Myocardial Infarction by Isoproterenol.. J Arak Uni Med Sci 2019; 22 (3) :118-128
URL: http://jams.arakmu.ac.ir/article-1-5950-en.html
1- Department of Physical Education, Mahallat Branch, Islamic Azad University, Mahallat, Iran.
2- Department of Physical Education, Mahallat Branch, Islamic Azad University, Mahallat, Iran. , abedi@iaumahallat.ac.ir
3- Department of Physiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
4- Department of Exercise Physiology, Faculty of Sport Sciences, Arak University, Arak, Iran.
Abstract:   (2395 Views)
Background and Aim: Extensive prevalence of myocardial infraction, and an increase of 36% in total deaths due to it by 2020, and attention to the causes and methods of alternative therapy is very important. There are different ways in treating these patients that endurance training is one of them. The aim of this study was to evaluate the effect of 8 weeks of incremental endurance training on serum levels of TSP-1 and MMP-1 in male Wistar rats with myocardial infarction.
Materials and Methods: In this controled experimental study with control group, 20 rats weighing 230 ±30 g (8-week-old) were randomly divided into incremental endurance training and control, after induction of infarction with Isoproteronol (150 mg/kg). The training group performed an 8-week training session (3 sessions a week, for 20 to 50 minutes at 12 to 18 meters per minute).Then, 24 hours later, the venous blood sample was collected to evaluate serum concentrations of TSP-1 and MMP-1 and transferred to the laboratory. Independent t-test was used to analyze the data at a significant level of p <0.05 to Graphed Pad software.
Ethical Considerations: This study with research ethics code IR.IAU.ARAK.REC.1397.007 was approved in Research Ethics Committee of Islamic Azad University, Arak branch, Iran.
Findings: The results showed that endurance training significantly increased serum MMP1 levels (p = 0.048) in rats with myocardial infraction, while there was no significant effect (p = 0.092) on the exercise program in serum TSP-1 levels.
Conclusion: the result of this study suggests that increasing endurance training may increase MMP-1 in rats with myocardial infraction, which can play an important role in angiogenesis and replacement of capillaries.
Full-Text [PDF 670 kb]   (964 Downloads)    
Type of Study: Original Atricle | Subject: Cardiology
Received: 2018/11/20 | Accepted: 2019/05/29

1. Zhang R, Lan C, Pei H, Duan G, Huang L, Li L. Expression of circulating miR-486 and miR-150 in patients with acute myocardial infarction. BMC cardiovascular disorders. 2015; 15(1):51.
2. Tomanek RJ. Response of the coronary vasculature to myocardial hypertrophy. Journal of the American College of Cardiology. 1990; 15(3):528-33.
3. Nourshahi M, Hedayati M, Nemati J, Ranjbar K, Gholamali M. Effect of 8 weeks endurance training on serum vascular endothelial growth factor and endostatin in wistar rats. Koomesh. 2012; 13(4):474-9.
4. Yu SP, Wei Z, Wei L. Preconditioning strategy in stem cell transplantation therapy. Translational stroke research. 2013; 4(1):76-88.
5. Schupp D, Huck B, Sykora J, Flechtenmacher C, Gorenflo M, Koch A, et al. Right ventricular expression of extracellular matrix proteins, matrix-metalloproteinases, and their inhibitors over a period of 3 years after heart transplantation. Virchows Archiv. 2006; 448(2):184-94.
6. Zhang H-y, Bao S-m, Shou W-l, Luan H-x, Zhang Y, Feng X, et al. Expression of matrix metalloproteinase-1 mRNA in peripheral blood mononuclear cells of systemic lupus erythematosus patients and its relationship with atherosclerosis. Chinese medical journal. 2009; 122(21):2593-7.
7. Almeida I, Oliveira AG, Lima M, Silva I, Vasconcelos C. Different contributions of angiostatin and endostatin in angiogenesis impairment in systemic sclerosis: a cohort study. Clinical and experimental rheumatology. 2016; 34(5):37-42.
8. Bornstein P. Thrombospondins: structure and regulation of expression. The FASEB Journal. 1992; 6(14):3290-9.
9. Malek MH, Olfert IM. Global deletion of thrombospondin‐1 increases cardiac and skeletal muscle capillarity and exercise capacity in mice. Experimental physiology. 2009; 94(6):749-60.
10. Kivelä R, Silvennoinen M, Lehti M, Jalava S, Vihko V, Kainulainen H. Exercise-induced expression of angiogenic growth factors in skeletal muscle and in capillaries of healthy and diabetic mice. Cardiovascular diabetology. 2008; 7(1):13.
11. Olfert IM, Breen EC, Gavin TP, Wagner PD. Temporal thrombospondin-1 mRNA response in skeletal muscle exposed to acute and chronic exercise. Growth Factors. 2006; 24(4):253-9.
12. Hoier B, Nordsborg N, Andersen S, Jensen L, Nybo L, Bangsbo J, et al. Pro‐and anti‐angiogenic factors in human skeletal muscle in response to acute exercise and training. The Journal of physiology. 2012; 590(3):595-606.
13. Xu X, Wan W, Powers AS, Li J, Ji LL, Lao S, et al. Effects of exercise training on cardiac function and myocardial remodeling in post myocardial infarction rats. Journal of molecular and cellular cardiology. 2008; 44(1):114-22.
14. Donley DA, Fournier SB, Reger BL, DeVallance E, Bonner DE, Olfert IM, et al. Aerobic exercise training reduces arterial stiffness in metabolic syndrome. Journal of Applied Physiology. 2014; 116(11):1396-404.
15. Schadler KL, Thomas NJ, Galie PA, Bhang DH, Roby KC, Addai P, et al. Tumor vessel normalization after aerobic exercise enhances chemotherapeutic efficacy. Oncotarget. 2016; 7(40):65429.
16. Lloyd PG, Prior BM, Yang HT, Terjung RL. Angiogenic growth factor expression in rat skeletal muscle in response to exercise training. American Journal of Physiology-Heart and Circulatory Physiology. 2003; 284(5):H1668-H78.
17. Bertinchant J, Robert E, Polge A, Marty-Double C, Fabbro-Peray P, Poirey S, et al. Comparison of the diagnostic value of cardiac troponin I and T determinations for detecting early myocardial damage and the relationship with histological findings after isoprenaline-induced cardiac injury in rats. Clinica Chimica Acta. 2000; 298(1-2):13-28.
18. Lobo Filho HG, Ferreira NL, Sousa RBd, Carvalho ERd, Lobo PLD, Lobo Filho JG. Experimental model of myocardial infarction induced by isoproterenol in rats. Brazilian Journal of Cardiovascular Surgery. 2011; 26(3):469-76.
19. Sun Y, Qi G, Li D, Meng H, Zhu Z, Zhao Y, et al. Walnut (Juglans regia L.) Kernel Extracts Protect Against Isoproterenol-Induced Myocardial Infarction in Rats. Rejuvenation research. 2018.
20. Afzalpour ME, Yousefi MR, Eivari SHA, Ilbeigi S. Changes in blood insulin resistance, GLUT4 & AMPK after continuous and interval aerobic training in normal and diabetic rats. J App Pharm Sci. 2016; 6:76-81.
21. Carmeli E, Haimovitch TG. The expression of MMP-2 following immobilization and high-intensity running in plantaris muscle fiber in rats. The Scientific World Journal. 2006; 6:542-50.
22. Rivilis I, Milkiewicz M, Boyd P, Goldstein J, Brown MD, Egginton S, et al. Differential involvement of MMP-2 and VEGF during muscle stretch-versus shear stress-induced angiogenesis. American Journal of Physiology-Heart and Circulatory Physiology. 2002.
23. Suhr F, Rosenwick C, Vassiliadis A, Bloch W, Brixius K. Regulation of extracellular matrix compounds involved in angiogenic processes in short‐and long‐track elite runners. Scandinavian journal of medicine & science in sports. 2010; 20(3):441-8.
24. Urso ML, Pierce JR, Alemany JA, Harman EA, Nindl BC. Effects of exercise training on the matrix metalloprotease response to acute exercise. European journal of applied physiology. 2009; 106(5):655-63.
25. Kwak H-B, Kim J-h, Joshi K, Yeh A, Martinez DA, Lawler JM. Exercise training reduces fibrosis and matrix metalloproteinase dysregulation in the aging rat heart. The FASEB Journal. 2011; 25(3):1106-17.
26. Tunc-Ata M, Mergen-Dalyanoglu M, Turgut S, Turgut G. Effect of acute and chronic exercise on plasma matrix metalloproteinase and total antioxidant levels. Journal of exercise rehabilitation. 2017; 13(5):508.
27. Puhl S-L, Müller A, Wagner M, Devaux Y, Bohm M, Wagner DR, et al. Exercise limits scar thinning after myocardial infarction in mice. American Journal of Physiology-Heart and Circulatory Physiology. 2015.
28. Nicosia RF, Tuszynski GP. Matrix-bound thrombospondin promotes angiogenesis in vitro. The Journal of cell biology. 1994; 124(1):183-93.
29. Kivelä R, Silvennoinen M, Touvra A-M, Lehti TM, Kainulainen H, Vihko V, et al. Effects of experimental type 1 diabetes and exercise training on angiogenic gene expression and capillarization in skeletal muscle. The FASEB journal. 2006; 20(9):1570-2.
30. Stenina OI, Krukovets I, Wang K, Zhou Z, Forudi F, Penn MS, et al. Increased expression of thrombospondin-1 in vessel wall of diabetic Zucker rat. Circulation. 2003; 107(25):3209-15.

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