Volume 22, Issue 3 (8-2019)
J Arak Uni Med Sci 2019, 22(3): 95-106 |
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Sanadgol N, Sharifzadeh M, Maleki P. Evaluation of Effects of Benzoaric Acid on the I-KB/NF-Kb Complex and Expression of Caspase-8 and -9 in Animal Model of Multiple Sclerosis. J Arak Uni Med Sci 2019; 22 (3) :95-106
URL: http://jams.arakmu.ac.ir/article-1-6028-en.html
URL: http://jams.arakmu.ac.ir/article-1-6028-en.html
1- Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran. Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran, Iran. , n.sanadgol@uoz.ac.ir
2- Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3- Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
2- Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3- Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
Abstract: (2697 Views)
Background and Aim: Regarding the importance of new treatments to control and treat multiple sclerosis (MS), in this study we investigated the role of Benzoaric acid (BA) on the neuro-inflammation and apoptosis processes in the cuprizone (cup)-induced animal model of MS.
Materials and Methods: In this experimental study, 35 males C57BL/6 mice were divided into five groups. The study groups were included, control: received six weeks of normal powdered food beside intraperitoneal (i.p.) injection of BA solvent (100 µL per day PBS) for the last two weeks, cup: received six weeks of powdered food contains 0.2% cup beside i.p. injection of BA solvent for the last two weeks and cup-treatment: received six weeks of powdered food contains 0.2% cup beside i.p. injection of 20, 40 and 80 mg/kg BA for the last two weeks. Eventually, the medial corpus callosum area of the animal’s brain was evaluated via western blot and Real-Time PCR methods.
Ethical Considerations: Ethical points were observed according to the declaration of Helsinki and relevant code of ethics, regarding minimizing harms during animal experimentation (UOZ-GR-9517-13).
Findings: Molecular studies have shown that BA-80 decreased mRNA (p <0.01) and protein expression of NF-KB and consequently increased I-KB/NF-KB ratio (p <0.05) and decreased inflammation in compare to cup group. Moreover, BA-80 decreased caspase-9 mRNA (p<0.01) and caspase-8 mRNA (p <0.05) and subsequently increased caspase-8/caspase-9 ratio (p<0.01) and decreased apoptosis in compare to cup group.
Conclusion: The dose of 80 mg/ml BA via decreasing cup-induced neuro-inflammation and neuro-apoptosis has protective effects in this model.
Materials and Methods: In this experimental study, 35 males C57BL/6 mice were divided into five groups. The study groups were included, control: received six weeks of normal powdered food beside intraperitoneal (i.p.) injection of BA solvent (100 µL per day PBS) for the last two weeks, cup: received six weeks of powdered food contains 0.2% cup beside i.p. injection of BA solvent for the last two weeks and cup-treatment: received six weeks of powdered food contains 0.2% cup beside i.p. injection of 20, 40 and 80 mg/kg BA for the last two weeks. Eventually, the medial corpus callosum area of the animal’s brain was evaluated via western blot and Real-Time PCR methods.
Ethical Considerations: Ethical points were observed according to the declaration of Helsinki and relevant code of ethics, regarding minimizing harms during animal experimentation (UOZ-GR-9517-13).
Findings: Molecular studies have shown that BA-80 decreased mRNA (p <0.01) and protein expression of NF-KB and consequently increased I-KB/NF-KB ratio (p <0.05) and decreased inflammation in compare to cup group. Moreover, BA-80 decreased caspase-9 mRNA (p<0.01) and caspase-8 mRNA (p <0.05) and subsequently increased caspase-8/caspase-9 ratio (p<0.01) and decreased apoptosis in compare to cup group.
Conclusion: The dose of 80 mg/ml BA via decreasing cup-induced neuro-inflammation and neuro-apoptosis has protective effects in this model.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2019/02/19 | Accepted: 2019/06/19
Received: 2019/02/19 | Accepted: 2019/06/19
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