Volume 27, Issue 3 (7-2024)
J Arak Uni Med Sci 2024, 27(3): 137-145 |
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Keykhapour M, Baharara J, Hatami H, Lotfi M, Farrokhyar S. Assessment of the Anti-Cancer Properties of Harmine and Low-Frequency Electromagnetic Field (50 Hz) on Ovarian Cancer Cells (A2780). J Arak Uni Med Sci 2024; 27 (3) :137-145
URL: http://jams.arakmu.ac.ir/article-1-7674-en.html
URL: http://jams.arakmu.ac.ir/article-1-7674-en.html
1- Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Mashhad, Iran
2- Department of Biology and Research Center for Animal Development Applied Biology, Mashhad Branch Islamic Azad University, Mashhad, Iran ,baharara@yahoo.com
3- Department of Hematology, Samen- Al- Aimah Hospital, North Khorasan Social Security Organization, Bojnurd, Iran
4- Department of Biology and Research Center for Animal Development Applied Biology, Mashhad Branch Islamic Azad University, Mashhad, Iran
2- Department of Biology and Research Center for Animal Development Applied Biology, Mashhad Branch Islamic Azad University, Mashhad, Iran ,
3- Department of Hematology, Samen- Al- Aimah Hospital, North Khorasan Social Security Organization, Bojnurd, Iran
4- Department of Biology and Research Center for Animal Development Applied Biology, Mashhad Branch Islamic Azad University, Mashhad, Iran
Abstract: (414 Views)
Introduction: Harmine is an alkaloid from the carboline family, belonging to the harmal plant, which has extensive applications in traditional medicine, with numerous studies highlighting its anti-cancer effects. Since biological processes are influenced by electromagnetic fields, the current study examined the anti-cancer effects of harmine and low-frequency electromagnetic fields on the expression of COX2, VEGF, and MMP-2 genes in the A2780 cell line.
Methods: In this experimental-laboratory study, ovarian cancer cell lines were randomly divided into four groups: control, harmine at concentrations of 6, 12, 24, 48, 96, and 192 micromolar, low-frequency electromagnetic field with an intensity of 50 Gauss, and harmine at a concentration of 48 micromolar with a low-frequency electromagnetic field of 50 Gauss intensity. Their toxicity was assessed using the MTT assay, nuclear morphological changes by DAPI staining, apoptotic effects of these compounds by measuring nitric oxide (NO), and gene expression changes by Real-Time PCR. Quantitative data were analyzed the ANOVA statistical test at a P < 0.05 level.
Results: Quantitative data comparison of this research indicated that harmine and a low-frequency electromagnetic field with an intensity of 50 Gauss caused a concentration-dependent reduction in the viability of ovarian cancer cells. Additionally, in the nitric oxide test, a significant decrease was found in the control group compared to the groups treated with a concentration of 48 micromolar and synergized with a 50 Gauss electromagnetic field (p<0.05). The expression of the aforementioned genes in treated cells showed a significant decrease. Treating ovarian cancer cells with harmine and its combined application led to significant nuclear morphological changes, including chromatin condensation, formation of apoptotic bodies, and wrinkling of the cell membrane.
Conclusions: The combined application of harmine and a low-frequency electromagnetic field induced apoptosis in A2780 cancer cells and resulted in the downregulation of COX2, VEGF-A, and MMP-2 gene expression. Consequently, the combined use of harmine with a low-frequency electromagnetic field, due to its effective cytotoxicity in inhibiting proliferation and inducing apoptosis, could be a suitable candidate for clinical studies.
Methods: In this experimental-laboratory study, ovarian cancer cell lines were randomly divided into four groups: control, harmine at concentrations of 6, 12, 24, 48, 96, and 192 micromolar, low-frequency electromagnetic field with an intensity of 50 Gauss, and harmine at a concentration of 48 micromolar with a low-frequency electromagnetic field of 50 Gauss intensity. Their toxicity was assessed using the MTT assay, nuclear morphological changes by DAPI staining, apoptotic effects of these compounds by measuring nitric oxide (NO), and gene expression changes by Real-Time PCR. Quantitative data were analyzed the ANOVA statistical test at a P < 0.05 level.
Results: Quantitative data comparison of this research indicated that harmine and a low-frequency electromagnetic field with an intensity of 50 Gauss caused a concentration-dependent reduction in the viability of ovarian cancer cells. Additionally, in the nitric oxide test, a significant decrease was found in the control group compared to the groups treated with a concentration of 48 micromolar and synergized with a 50 Gauss electromagnetic field (p<0.05). The expression of the aforementioned genes in treated cells showed a significant decrease. Treating ovarian cancer cells with harmine and its combined application led to significant nuclear morphological changes, including chromatin condensation, formation of apoptotic bodies, and wrinkling of the cell membrane.
Conclusions: The combined application of harmine and a low-frequency electromagnetic field induced apoptosis in A2780 cancer cells and resulted in the downregulation of COX2, VEGF-A, and MMP-2 gene expression. Consequently, the combined use of harmine with a low-frequency electromagnetic field, due to its effective cytotoxicity in inhibiting proliferation and inducing apoptosis, could be a suitable candidate for clinical studies.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2024/03/17 | Accepted: 2024/07/7
Received: 2024/03/17 | Accepted: 2024/07/7
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