Volume 28, Issue 4 (10-2025)
J Arak Uni Med Sci 2025, 28(4): 246-252 |
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Fatemi Nejad R, Dayer M R, Dorostghoal M, Parishani M R. Effects of Intraperitoneal Injection of Botox on Biochemical and Structural Parameters of the Kidneys of Female Wistar Rats. J Arak Uni Med Sci 2025; 28 (4) :246-252
URL: http://jams.arakmu.ac.ir/article-1-7921-en.html
URL: http://jams.arakmu.ac.ir/article-1-7921-en.html
1- Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran ,mrdayer@scu.ac.ir
2- Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran ,
Abstract: (377 Views)
Introduction: Botulinum neurotoxin protein (Botox) is widely used for cosmetic purposes to reduce facial wrinkles without regard to its side effects. The present study was conducted to investigate the effects of Botox on blood biochemical factors and the kidney tissue structure of female rats.
Methods: In this study, 28 adult female Wistar rats were selected and randomly divided into four groups (n = 7). The first three groups received intraperitoneal injections of Botox (in the lower third of the abdomen, one centimeter below and to the left of the navel) at doses of 4, 6, and 8 units, respectively, while the fourth group (the control group) received a saline injection. Then the animals were kept in the animal house for three months with full access to food and water at a temperature of 22°C and a 12-hour light-dark cycle. Following a 24-hour fasting period at the end of the study, blood samples were collected via cardiac puncture under ether anaesthesia. Then, the animals were euthanized without pain, and their kidney tissue samples were extracted for histological examination.
Results: Biochemical results indicated a significant increase in serum creatinine levels following injection with 6 and 8 units of Botox, indicating renal impairment. Although the increase in the amount of serum uric acid levels compared to the control group did not reach statistical significance at the 95% confidence level (P < 0.05), it showed significance at the 94% level (p < 0.06), suggesting primary renal damage. Histopathological examination demonstrated that intraperitoneal injection of 8 units of Botox induced scattered and limited structural alterations in renal tissue, including vacuolar degeneration of tubular epithelial cells.
Conclusions: The results of this research show that the long-term and repeated use of Botox may cause significant renal damage in addition to local lesions and threaten a person's life.
Methods: In this study, 28 adult female Wistar rats were selected and randomly divided into four groups (n = 7). The first three groups received intraperitoneal injections of Botox (in the lower third of the abdomen, one centimeter below and to the left of the navel) at doses of 4, 6, and 8 units, respectively, while the fourth group (the control group) received a saline injection. Then the animals were kept in the animal house for three months with full access to food and water at a temperature of 22°C and a 12-hour light-dark cycle. Following a 24-hour fasting period at the end of the study, blood samples were collected via cardiac puncture under ether anaesthesia. Then, the animals were euthanized without pain, and their kidney tissue samples were extracted for histological examination.
Results: Biochemical results indicated a significant increase in serum creatinine levels following injection with 6 and 8 units of Botox, indicating renal impairment. Although the increase in the amount of serum uric acid levels compared to the control group did not reach statistical significance at the 95% confidence level (P < 0.05), it showed significance at the 94% level (p < 0.06), suggesting primary renal damage. Histopathological examination demonstrated that intraperitoneal injection of 8 units of Botox induced scattered and limited structural alterations in renal tissue, including vacuolar degeneration of tubular epithelial cells.
Conclusions: The results of this research show that the long-term and repeated use of Botox may cause significant renal damage in addition to local lesions and threaten a person's life.
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2025/01/3 | Accepted: 2025/05/11
Received: 2025/01/3 | Accepted: 2025/05/11
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