Volume 28, Issue 4 (10-2025)
J Arak Uni Med Sci 2025, 28(4): 0-0 |
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Mohammadifar M, Bahmani F, Abed A, Hamidi G, Aarabi M H, Talaei S A. Nanoemulsion Containing Essential Oils of Rosemary and Peppermint Reduces Neuropathic Pain in Rats with Chronic Constriction Injury of the Sciatic Nerve. J Arak Uni Med Sci 2025; 28 (4)
URL: http://jams.arakmu.ac.ir/article-1-8061-en.html
URL: http://jams.arakmu.ac.ir/article-1-8061-en.html
Mojgan Mohammadifar1 
, Fereshteh Bahmani2 , Alireza Abed1 , Gholamali Hamidi1 , Mohammad Hossein Aarabi3 , Sayyed Alireza Talaei4
, Fereshteh Bahmani2 , Alireza Abed1 , Gholamali Hamidi1 , Mohammad Hossein Aarabi3 , Sayyed Alireza Talaei4
1- Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran
2- Biochemistry and Nutrition in Metabolic Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran
3- Department of Clinical Biochemistry, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
4- Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran ,talaei@kaums.ac.ir
2- Biochemistry and Nutrition in Metabolic Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran
3- Department of Clinical Biochemistry, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
4- Physiology Research Center, Kashan University of Medical Sciences, Kashan, Iran ,
Abstract: (70 Views)
Introduction: The analgesic effects of rosemary and peppermint essential oils have been shown in many studies. Low solubility of essential oils in water and low bioavailability limit their consumption, and nanoparticles can help solve these limitations. This study aimed to investigate the analgesic effect of nanoemulsion containing rosemary and peppermint essential oils in a rat model of neuropathic pain.
Methods: Forty male rats were assigned to four groups: control (CON), neuropathic pain received normal saline (NPS), nanoemulsion (NPN), or gabapentin (NPG) for 21 days. Neuropathic pain was induced using the CCI model. Animals received a nanoemulsion containing 10% rosemary extract and peppermint essential oil via gavage. Behavioral tests (Thermal Hyperalgesia, Thermal Allodynia, and Mechanical Allodynia) were done on days 1, 4, 7, 14, and 21 of the study. Biochemical tests were also done on the sciatic nerve of animals at the end of the study.
Results: The NPS rats had a significantly increased pain threshold compared to the control group (P<0.001). Furthermore, the analgesic potential of nanoemulsion containing rosemary and peppermint essential oils was seen in allodynia and hyperalgesia tests. Biochemical analyses showed that CCI reduces total antioxidant capacity, superoxide dismutase, and glutathione peroxidase activity, and also increases MDA levels in the rats' sciatic nerve (P < 0.001 for all comparison). Administering nanoemulsion containing rosemary and peppermint essential oils reversed all the above-mentioned changes in the biochemical parameters (P < 0.001).
Conclusions: Our findings revealed that treating rats with nanoemulsion containing rosemary and peppermint essential oils can reduce neuropathic pain symptoms by balancing their sciatic nerve's oxidant and antioxidant systems.
Methods: Forty male rats were assigned to four groups: control (CON), neuropathic pain received normal saline (NPS), nanoemulsion (NPN), or gabapentin (NPG) for 21 days. Neuropathic pain was induced using the CCI model. Animals received a nanoemulsion containing 10% rosemary extract and peppermint essential oil via gavage. Behavioral tests (Thermal Hyperalgesia, Thermal Allodynia, and Mechanical Allodynia) were done on days 1, 4, 7, 14, and 21 of the study. Biochemical tests were also done on the sciatic nerve of animals at the end of the study.
Results: The NPS rats had a significantly increased pain threshold compared to the control group (P<0.001). Furthermore, the analgesic potential of nanoemulsion containing rosemary and peppermint essential oils was seen in allodynia and hyperalgesia tests. Biochemical analyses showed that CCI reduces total antioxidant capacity, superoxide dismutase, and glutathione peroxidase activity, and also increases MDA levels in the rats' sciatic nerve (P < 0.001 for all comparison). Administering nanoemulsion containing rosemary and peppermint essential oils reversed all the above-mentioned changes in the biochemical parameters (P < 0.001).
Conclusions: Our findings revealed that treating rats with nanoemulsion containing rosemary and peppermint essential oils can reduce neuropathic pain symptoms by balancing their sciatic nerve's oxidant and antioxidant systems.
Keywords: Neuropathic pain, Nanoemulsion, Essential oil, Rosmarinus officinalis L., Mentha piperita, Rats
Type of Study: Original Atricle |
Subject:
Basic Sciences
Received: 2025/07/12 | Accepted: 2025/07/27
Received: 2025/07/12 | Accepted: 2025/07/27
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